Parallel between two high-efficiency transformation methods in yeast and screening for applicability of transformants

Wanda Smith Kendrick, Fayetteville State University

Abstract

Transformation of exogenous DNA into intact Saccharomyces cerevisiae cells has been accomplished by using the alkali cations method and the electroporation method. The addition of SSDNA and PEG has enhanced transformation efficiency in both methods. In this study, transformation efficiencies from published documents using LiAc competent cells (yeast strains, LP2752-4B and RS109-2A) is compared to data collected using LiCsAc competent cells and electroporation. Results show that transformation efficiency using LiCsAc is comparable to that of documented data using LiAc for both strains. Strain RS109-2A may be less suitable for electroporation while electroporation of LP2752-4B is comparable to that of the LiCsAc method but less than that for the LiAc method. ^

Subject Area

Biology, Molecular|Biology, Cell

Recommended Citation

Wanda Smith Kendrick, "Parallel between two high-efficiency transformation methods in yeast and screening for applicability of transformants" (January 1, 1997). ETD Collection for Fayetteville State University. Paper AAI1390615.
http://digitalcommons.uncfsu.edu/dissertations/AAI1390615

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