Examination of the putative antimutagenic effect of selenium compounds on in vitro DNA synthesis by E. coli DNA polymerase I
Selenium compounds are recognized as being both anticarcinogenic and toxic. In this research project the antimutagenic effects of SeCl 4 during in vitro gap-filling DNA synthesis are being analyzed using the M13mp2 Lac Z ∝-complementation assay. In vitro DNA synthesis reactions catalyzed by exonuclease deficient E. coli DNA polymerase I (Klenow fragment) were carried out under various experimental conditions including varying concentrations of SeCl 4 (0 to 4.0 mM) and deoxynucleotide (dNTP) pool bias. SeCl4 concentrations higher than 0.2 mM inhibited DNA synthesis and caused apparent DNA degradation. For reactions showing complete DNA synthesis, there was not a decrease in mutational frequency compared to the control containing no SeCl 4, using either balanced dNTP concentrations or dNTP pool bias conditions. In contrast to SeCl4, seleno-L-methionine did not inhibit DNA synthesis and showed a slight but inconsistent decrease in mutation frequency. These data indicate that the selenium compounds tested are not antimutators during in vitro DNA synthesis. ^
Snead, Kimberly Neshell, "Examination of the putative antimutagenic effect of selenium compounds on in vitro DNA synthesis by E. coli DNA polymerase I" (1999). ETD Collection for Fayetteville State University. AAI1406426.