Examination of the putative antimutagenic effect of selenium compounds on in vitro DNA synthesis by Escherichia coli DNA polymerase I
Selenium compounds are recognized as being both anticarcinogenic and toxic. In this research project the antimutagenic effects of SeCl 4 during in vitro gap-filling DNA synthesis are being analyzed using the M13mp2 Lac Z ∝-complementation assay. In vitro DNA synthesis reactions catalyzed by exonuclease deficient E. coli DNA polymerase I (Klenow fragment) were carried out under various experimental conditions including varying concentrations of SeCl 4 (0 to 4.0 mM) and deoxynucleotide (dNTP) pool bias. SeCl4 concentrations higher than 0.2 mM inhibited DNA synthesis and caused apparent DNA degradation. For reactions showing complete DNA synthesis, there was not a decrease in mutational frequency compared to the control containing no SeCl 4, using either balanced dNTP concentrations or dNTP pool bias conditions. In contrast to SeCl4, seleno-L-methionine did not inhibit DNA synthesis and showed a slight but inconsistent decrease in mutation frequency. These data indicate that the selenium compounds tested are not antimutators during in vitro DNA synthesis.
Snead, Kimberly Neshell, "Examination of the putative antimutagenic effect of selenium compounds on in vitro DNA synthesis by Escherichia coli DNA polymerase I" (1999). ETD Collection for Fayetteville State University. AAI1406426.