Metabolism of chlorpyrifos by Pseudomonas aeruginosa increases toxicity and acetylcholinester inhibition in adult zebrafish (danio rerio)
Chlorpyrifos (CPF) is a commonly used insecticide and belongs to a diverse family of chemicals called organophosphates (OPs). Due to the efficacy of OPs against crop pests, growers have used them on a large scale. Such broad use has resulted in concern about public health and impacts on the ecosystem. Bioremediation is one strategy that uses biological organisms to reduce toxicants in the environment. Toward that goal, the present study examined the efficacy of Pseudomonas aeruginosa, a specific bacterial species, in degrading CPF. Results showed that after 5 days P. aeruginosa degraded CPF completely to its primary metabolites, CPF-oxon and 3,5,6-trichloro-2pyridinol (TCP), based on High Performance Liquid Chromatography analysis. However, the resulting metabolites appeared to increase toxicity in zebrafish adults. Our findings suggest that Pseudomonas aeruginosa do not appear to serve as optimum bioremediation agents; rather, this species metabolizes CPF to more toxic metabolites, increasing mortality in fish.^
Health Sciences, Toxicology|Biology, Microbiology|Environmental Sciences
Kharabsheh, Hamzah Abdelraouf, "Metabolism of chlorpyrifos by Pseudomonas aeruginosa increases toxicity and acetylcholinester inhibition in adult zebrafish (danio rerio)" (2012). ETD Collection for Fayetteville State University. AAI1525802.