Chromatin dynamics is correlated with replication timing
Document Type
Article
Publication Date
4-8-2009
Abstract
Discrete chromatin domains (ChrD), containing an average of ?1 Mbp DNA, represent the basic structural units for the regulation of DNA organization and replication in situ. In this study, a bio-computational approach is employed to simultaneously measure the translational motion of large populations of ChrD in the cell nucleus of living cells. Both movement and configurational changes are strikingly higher in early S-phase replicating ChrD compared to those that replicate in mid and late S-phase. The chromatin dynamics was not sensitive to transcription inhibition by ?-amanitin but was significantly reduced by actinomycin D treatment. Since a majority of active genes replicate in early S-phase, our results suggest a correlation between levels of chromatin dynamics and chromatin poised for active transcription. Analysis of ChrD colocalization with transcription sites and cDNA with ChrD and transcription sites further supports this proposal. © Springer-Verlag 2009.
Recommended Citation
Pliss, Artem; Malyavantham, Kishore; Bhattacharya, Sambit; Zeitz, Michael; and Berezney, Ronald, "Chromatin dynamics is correlated with replication timing" (2009). College of Health, Science, and Technology. 1021.
https://digitalcommons.uncfsu.edu/college_health_science_technology/1021