Simultaneous enantioseparation and tandem UV-MS detection of eight ?-blockers in micellar electrokinetic chromatography using a chiral molecular micelle
Document Type
Article
Publication Date
3-15-2005
Abstract
The feasibility of using a new and more versatile polymeric chiral surfactant, i.e., poly(sodium N-undecenoxy carbonyl-L-leucinate (poly-L-SUCL) is investigated for simultaneous enantioseparation and detection of eight structurally similar ?-blockers with tandem UV and MS detection. Three optimization approaches, i.e., direct infusion-MS, capillary zone electrophoresis-MS, and chiral micellar electrokinetic chromatography-mass spectrometry (CMEKC-MS), were investigated to optimize sheath liquid parameters, spray chamber parameters, and CMEKC separation parameters for maximum sensitivity and chiral resolution. Compared to unpolymerized micelle of L-SUCL, the use of micelle polymer (i.e., poly-L-SUCL) provided significantly higher separation efficiency, lower separation current, and higher detection sensitivity for CMEKC-ESI-MS of ?-blockers. It was also observed that, unlike monomeric L-SUCL, polymeric L-SUCL provided enantioseparation of all ?-blockers even at the lowest surfactant concentration (i.e., 5 mM poly-L-SUCL). Under optimum CMEKC and ESI-MS conditions (15 mM poly-L-SUCL, 25 mM each of NH4OAc and TEA (pH 8.0); 80% (v/v) methanol sheath liquid containing 40 mM NH4OAc (pH 8.0); sheath liquid flow rate, 5.0 ?L/min; drying gas flow rate, 5 L/min; drying gas temperature, 200°C; nebulizing pressure, 6 psi (0.414 bar); capillary voltage, +2.5 kV; fragmenter voltage, 85 V), baseline enantioseparation of eight ?-blockers was achieved by tandem UV (in ?30 min) and MS (in ?60 min) detection. Calibration curves for all ?-blockers were linear in the range of 0.01-0.6 mM for both CMEKC-UV and CMEKC-MS methods, but the later method provided better concentration limit of detection with similar RSD for migration time and peak areas. The CMEKC-ESI-MS method appears suitable for use as a routine procedure for high-throughput separation of ?-blockers with high sensitivity. © 2005 American Chemical Society.
Recommended Citation
Akbay, Cevdet; Rizvi, Syed A.A.; and Shamsi, Shahab A., "Simultaneous enantioseparation and tandem UV-MS detection of eight ?-blockers in micellar electrokinetic chromatography using a chiral molecular micelle" (2005). College of Health, Science, and Technology. 146.
https://digitalcommons.uncfsu.edu/college_health_science_technology/146