Isoflavone biosynthetic pathways and methods of quantification

Document Type

Book

Publication Date

1-10-2022

Abstract

Phytoestrogens or isoflavones are secondary metabolites abundant in soybean and other legumes' seeds but also in several nonlegume plants. Among the most studies, isoflavones in recent decades are genistein, daidzein, and glycitein because of their pharmacological properties and benefits to human health. In this chapter, we summarized briefly isoflavone biosynthesis and methods of their extraction from seeds and other plant parts. Extraction methods include ultrasonification extraction, supercritical fluid extraction, soxhlet extraction, pressurized-liquid extraction, ultrasound-assisted extraction, and eco-friendly method of extraction. Quantification methods include the mostly used high-performance liquid chromatography (HPLC), gas chromatography (GC), liquid chromatography mass spectrometry (LC-MS), and few other methods.

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